Figure 8.
Altered corticostriatal short-term plasticity in Nrp2 CKO mice. A, Schematic illustrating the experimental paradigm where SPNs were recorded in coronal striatal slices. A bipolar stimulating electrode was placed in the corpus callosum to stimulate corticostriatal fibers. B, C, Representative examples of EPSPs measured in SPNs after a pair of electrical stimuli. ISIs: 20 ms in K; 100 ms in L (K1, L1: WT; K2 and L2: Nrp2 CKO mice). Note that in WT SPNs there is a strong paired-pulse facilitation that is impaired in Nrp2 CKO. D, E, Representative current-clamp traces showing corticostriatal EPSPs elicited by paired stimuli with increasing ISIs in WT (M) and Nrp2 CKO (N) mice. F, Box plots representing the paired-pulse ratio of the responses at different ISIs (20, 40, 60, 80, 100, 120, and 200 ms) for the two genotypes and unpaired t test was performed (***p = 0.0003, 20 ms; ****p < 0.0001, 40 ms; ***p = 0.0002, 60 ms; ****p < 0.0001, 80 ms; **p = 0.0016, 100 ms; **p = 0.0043, 120 ms; ****p < 0.0001, 200 ms comparison between WT and Nrp2−/−). Responses are expressed as the amplitude of the second EPSP divided by the amplitude of the first one. G, Summary graph of PPRs recorded from medium spiny neurons plotted against ISIs for cortical stimulation in WT (blue) and Nrp2 CKO (red).