Figure 4.
Chem-LTD treatment reduces the amount of AKAP150 and PKA-RII present in PSD fractions. A, Semiquantitative Western analysis shows a reduction in the amount of AKAP150 in the Triton-insoluble pellet after NMDA treatment (top panel). AKAP150 levels were determined to be 50.2 ± 4% of the control amount (bottom panel) (*p < 0.05; n = 4; t test). B, PKA-RIIβ also is reduced in the Triton-insoluble pellet (top panel) to 60.4 ± 4% of control (bottom panel) (*p < 0.05; n = 4; t test). C, NMDA treatment induces a similar loss in PKA-RIIα levels in the PSD fraction (top panel) to 74.9 ± 6% of control levels (bottom panel) (*p < 0.05; n = 4; t test). Data are expressed as mean ± SEM.