Fig. 3.
Opioid withdrawal enhances mIPSCs in neurons from dependent animals. A, Consecutive raw current traces of mIPSCs recorded from neurons maintained in morphine (5 μm) in the absence and presence of naloxone (1 μm). B, Cumulative distribution plots of mIPSC amplitude and frequency from the dependent neuron shown above inA for the presence of morphine (5 μm,solid line) and after superfusion of naloxone (1 μm, dashed line). C, Time course of normalized mIPSC frequency in neurons maintained in morphine (5 μm) during superfusion of naloxone (1 μm) in dependent (closed circles) and vehicle-treated (open circles) animals. Each point represents the mean (± SEM) of four to six neurons.