Table 2.
Quantitative evaluation of GAP-43 and L1.1 mRNA expression of different brain nuclei after proximal (level 1) and distal (level 2) lesion
| GAP-43 | L1.1 | |||||||
|---|---|---|---|---|---|---|---|---|
| Unlesioned control (n = 3 animals) | Level 1 lesion (n = 4 animals) | Level 2 lesion (n = 4 animals) | Induction index | Unlesioned control (n = 3 animals) | Level 1 lesion (n = 3 animals) | Level 2 lesion (n = 3 animals) | Induction index | |
| Brain nuclei responding similarly to proximal and distal lesion | ||||||||
| NMLF | 12.3 ± 11.67 | 110.0 ± 24.68 | 70.0 ± 20.72 | 1.3 | 3.3 ± 1.52 | 53.0 ± 12.66 | 51.3 ± 8.50 | 0.8 |
| IMRF | 9.6 ± 6.44 | 136.3 ± 46.32 | 83.0 ± 11.60 | 1.4 | 5.3 ± 4.73 | 64.7 ± 18.84 | 60.3 ± 14.98 | 0.9 |
| MaON | 8.0 ± 4.36 | 46.0 ± 14.10 | 48.5 ± 6.36 | 0.9 | 5.3 ± 3.50 | 34.3 ± 4.94 | 26.7 ± 8.14 | 1.2 |
| Brain nuclei responding differently to proximal and distal lesion | ||||||||
| NR | 1.7 ± 2.88 | 37.3 ± 10.80 | 7.3 ± 8.80 | 3.8 | 0.6 ± 1.14 | 23.7 ± 7.50 | 2.0 ± 1.73 | 8.9 |
| NLL | 2.0 ± 3.46 | 51.7 ± 8.46 | 8.0 ± 5.52 | 4.8 | 0.6 ± 1.14 | 23.7 ± 12.66 | 4.3 ± 1.52 | 4.1 |
| T | 4.0 ± 2.01 | 19.75 ± 25.26 | 1.5 ± 1.90 | 5.4 | 0.0 ± 0.00 | 2.0 ± 1.73 | 1.0 ± 1.00 | 0.8 |
Average numbers of cellular profiles showing high intensity of GAP-43 and L1.1 mRNA labeling are given (with SD). The induction index is a measure that compares the effectiveness of distal and proximal lesions to induce upregulation of a specific mRNA. It compensates for different numbers of neurons being axotomized after the two types of lesion (for calculation see Material and Methods). Abbreviations of brain nuclei are the same as in Table 1.