Table 1.
Primers used to construct CB1 cannabinoid receptor mutants
| Primer | Sequence (5′–3′) |
|---|---|
| CB1 sense | CGGGATCCATGAAGTCGATCCTAGATGGCTTG |
| CB1 antisense | CGGAATTCACAAAAGCAGCTCACAGAGC |
| Δ418 antisense | CGGAATTCTTATTCGCACGAAGGGAACATGCTTCG |
| Δ439 antisense | CGGAATTCTTAGTTGGCGTGCTTGTGCAG |
| Δ460 antisense | CGGAATTCTTACTTCGCGATCTTAACCGTGCTCTTGAT |
| T419A antisense | TAGAGGCTGTGCGGCTTCGCA |
| T419A sense | TGCGAAGGCGCCGCACAGCCTCTA |
| S426A antisense | GTGCTTGTGCAGGCAGTCTGAGTCCCC |
| S426A sense | GGGGACTCAGACTGCCTGCACAAGCAC |
| S430A antisense | GCAGTCTGCGTCCCCCATGCTGTT |
| S430A sense | AACAGCATGGGGGACGCAGACTGC |
| S426A/S430A antisense | GTCTGCGTCCCCCATGGCGTTGTCTAGAGGCTGTGCGGCGCC |
| S426A/S430A sense | GGCGCCGCACAGCCTCTAGACAACGCCATGGGGGACGCAGACGAC |
| ΔG418-N438 antisense | TTCGCACGAAGGGAACATGC |
| ΔG418-N438 sense | ATGTTCCCTTCGTGCGAAAACACAGCCAGCATGCACAGG |
Sense refers to the forward primer, and antisense to the reverse primer for each reaction.