Fig. 4.
The effect of MTSEA-Biotin, applied in the presence and absence of GABA, on the subsequent GABA-activated currents of α1F64Cβ2 receptors. Current traces recorded by two-electrode voltage clamping of a single oocyte are shown. The current responses to applications of 10 mm GABA (horizontal bars) were recorded subsequent to the application of the following sequence of solutions (arrows): buffer, 50 μm MTSEA-Biotin + 300 mm GABA (MTS+GABA, 30 sec), 50 μm MTSEA-Biotin (MTS, 30 sec), 20 mm dithiothreitol (DTT, 2 min), and 50 μm MTSEA-Biotin (MTS, 30 sec). After all GABA applications, a 10 min wash in ND96 buffer occurred before any reagent application. The results demonstrate that the effects of MTSEA-Biotin on α1F64Cβ2 receptors are protectable by GABA, recoverable by DTT treatment, and repeatable.