Fig. 9.
βA and PrP fibrils stimulate a respiratory burst in THP-1 cells. THP-1 cells were incubated in DMSO vehicle (control), 50 nm thapsigargin (Thaps), 5 μmPP1, or 2 μmGo6976 (30 min; 37°C for each) before incubation in PrP 106–126 (40 μm) or βA 25–35 (40 μm). Cells were stimulated for 30 min in HBSS containing 1 μg/ml NBT. Cells were collected and sonicated in RIPA buffer. Generation of superoxide anion was measured by the change in absorbance at 550 nm. The mean absorbance values (± SEM) representative of three independent experiments are shown.