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. 2019 Jul 23;216(10):2348–2361. doi: 10.1084/jem.20190701

Figure 3.

Figure 3.

α/β-Tryptase but not β-tryptase makes human MCs susceptible to vibration-triggered degranulation. (A) Treatment of skin MCs adherent to dermatan sulfate, but not to chondroitin sulfate A, degranulate when vibrated if pretreated with α/β-tryptase Hi (3.5 µg/ml), but not with α/β-tryptase Lo (0.35 µg/ml) or with β-tryptase at either concentration. Pretreatment of α/β-tryptase Hi with B12 anti-tryptase IgG, but not with SBTI or B2 anti-tryptase IgG, prevents this degranulation. Triplicate data with cells from one subject are shown, representative of two independent experiments. GAG, glycosaminoglycan; DS, dermatan sulfate; CSA, chondroitin sulfate A. (B) Time for incubating α/β-tryptase (3.5 µg/ml) with skin MCs to make them susceptible to vibration-triggered degranulation during the final 20 min of incubation. Triplicate data with cells from one subject are shown, representative of two independent experiments. *, P < 0.05; ***, P < 0.001; ANOVA followed by a Holm–Sidak test compared with the buffer control. Bars show means, and error bars show the SD.