Fig. 2.
Effect of 16α-iodo-17β-estradiol on ERK phosphorylation in wild-type cerebral cortex. The ER-α-selective ligand 16α-iodo-17β-estradiol was evaluated for its ability to phosphorylate ERK [using the dual phospho-specific ERK antibody (New England Biolabs)]. At concentrations known to selectively bind ER-α, this ligand failed to elicit ERK phosphorylation. The bottom panel represents reprobing of the phosphoERK blot for ERK protein to verify equal loading of protein across lanes. Densitometric representation of the relative intensities of ERK1 phosphorylation is also provided, demonstrating significant inhibition of ERK1 phosphorylation in response to 16α-iodo-17β-estradiol. Data are representative of three independent experiments.