Fig. 2.
Disrupting the actin cytoskeleton disperses the large α7-nAChR clusters. E15 ciliary neurons were treated with (A, B, F) or without (C, D; control) latrunculin A for 4 hr and then labeled for F-actin with rhodamine–phalloidin (A, C) and for α7-nAChRs with Alexa488–αBgt (B, D–F). Vertical pairs of panels represent the same cell imaged with confocal fluorescence microscopy either immediately (A, B;C, D) or before and after a 4 hr exposure to latrunculin A (E, F). C,D, The corresponding time control (i.e., the same 4 hr in the absence of latrunculin A) is shown. The latrunculin A treatment caused the loss of F-actin and dispersed the large α7-nAChR clusters into numerous microclusters of receptors distributed over the cell body. Scale bar, 10 μm.