Fig. 6.
PPARγ agonists inhibit IL-6 and TNFα gene expression. THP-1 cells were transiently transfected with TNFα–luciferase reporter (A, B) or IL-6–luciferase reporter (C, D) constructs and assayed for promoter activity 48 hr later. The cells were cotransfected with a β-galactosidase–reporter construct to control for transfection efficiency. During the last 4 hr the cells were incubated alone (blackbars), with fibrillar Aβ1–40 (40 μm;stripedbars;B, D) or Aβ25–35 (60 μm;speckledbars; A, C), or with 5 μg/ml LPS (positive control; open bars) in the presence or absence of troglitazone (20 μm), ciglitizone (50 uM), DHA (100 μm), 15d-PGJ2 (50 μm), ibuprofen (3 mm), or indomethacin (200 μm). The data shown represent the average (± SEM) of three independent experiments. Unpaired ANOVA was performed with Tukey-Kramer post comparison to evaluate statistical significance (* =p < 0.001).