Fig. 7.
MMP-9 promoter activity is modulated by ER-α. ER-negative SK-N-BE cells were transiently transfected with the 2200 bp (A) or 670 bp (B) fragments of the hMMP-9 promoter, in the absence or presence of hER-α expression vector. Cells were treated with 10 nm PMA and/or 10 nm 17β-estradiol (E2), as specified aboveeach figure. In B, 0.01, 0.1, 1, and 10 nm 17β-estradiol were assayed. The reported promoter activity is calculated by normalizing the CAT level with protein content and represents the mean ± SD of triplicate determinations, representative of at least three separate experiments. °p < 0.01, as compared with the control, i.e., untreated cells without transfected ER-α, *p < 0.05, as compared with the LPS value of the same experimental group, and ··p < 0.05, as compared with ER-α-transfected cells without treatment, were calculated by ANOVA test, followed by a Bonferroni analysis.