Table 1.
Summary of desensitization and deactivation kinetics
| Construct | % τfast | τfast(msec) | # fast/total | Extent (%) | τdeact. (msec) |
|---|---|---|---|---|---|
| αβδ | 2.4 ± 2.4 | – | 1 /11 | 12.4 ± 4.8 | 54.7 ± 4.8 |
| δ-γM2e | 6.1 ± 4.5 | – | 1 /10 | 17.8 ± 6.5 | 45.4 ± 11.3 |
| δ-γM1i | 9.6 ± 3.8 | – | 6 /26 | 33.1 ± 5.2* | 54.6 ± 8.1 |
| δ-γM1p | 8.2 ± 8.2 | – | 1 /6 | 14.7 ± 11.9 | 10.3 ± 2.2* |
| δ-γM1pre-iso | 3.5 ± 3.5 | – | 1 /14 | 15.8 ± 5.8 | 27.4 ± 4.9* |
| δ-γM1e | 40.8 ± 6.9 | 11.2 ± 2.8 | 13 /14 | 69.3 ± 7.5 | 107.5 ± 14.7* |
| αβγ | 41.3 ± 3.9 | 6.0 ± 0.7 | 13 /13 | 67.4 ± 3.8 | 171.8 ± 20.4 |
| δ(M2S) | 9.0 ± 4.8 | – | 3 /12 | 26.3 ± 8.4 | 61.7 ± 12.0 |
| γ(M2S) | 45.4 ± 2.2 | 7.9 ± 1.3 | 9 /9 | 69.6 ± 3.0 | 137.8 ± 24.6 |
| γ2L(YΔV) | 45.2 ± 7.8 | 6.9 ± 1.4 | 7 /7 | 68.1 ± 6.3 | 79.3 ± 21.8 |
| γ2L(FΔY) | 34.2 ± 5.2 | 8.0 ± 1.7 | 8 /8 | 57.7 ± 6.7 | 86.6 ± 18.7 |
| γ2L(YFΔVY) | 40.5 ± 5.9 | 9.0 ± 1.1 | 17 /19 | 67.5 ± 4.4 | 39.7 ± 4.7* |
| α(YFΔVY) | 18.5 ± 5.7* | 11.6 ± 1.7 | 6 /9 | 64.6 ± 3.6 | 56.5 ± 8.3 |
| β(YFΔVY) | 30.4 ± 4.8 | 7.8 ± 0.9 | 12 /14 | 61.5 ± 4.4 | 99.2 ± 12.5* |
| δ(VYΔYF) | 35.3 ± 3.6 | 9.0 ± 1.1 | 13 /21 | 38.4 ± 6.3* | 76.5 ± 6.0 |
| γ-δ M1e | 40.1 ± 9.2 | 11.8 ± 1.9 | 6 /7 | 56.2 ± 10.3 | 68.4 ± 11.4 |
| γ-δ M1i | 40.6 ± 5.7 | 10.5 ± 1.9 | 8 /8 | 66.8 ± 7.3 | 186.0 ± 29.0 |
% is the relative contribution of fast desensitization (see Results). τfast is shown only for constructs that had fast desensitization.
fast/total is the number of patches having fast desensitization/total patches studied. Extent (%) refers to the extent of current loss over the 400 msec GABA pulse (see Materials and Methods). τdeact is the weighted deactivation rate (see Materials and Methods). Asterisks indicate significant differences from both αβδ and αβγ isoforms. Data are mean ± SEM.