Figure 1. The Compartmentalization of IL-1R1 Expression in the Brain.
(A) A representative Tile Scan image of RFP labeling of hippocampal sections from Il1r1GR/GR mice. Labeling of cell-type-specific IL-1R1s is marked with dashed squares according to their morphological features and shown at higher magnification in insets a–e. Scale bar, 200 μm.
(B) PCR detection of restored amplicon in hippocampal DNA from cell-type-specific IL-1R1 lines.
(C) Hippocampal IL-1R1 mRNA expression in cell-type-specific IL-1R1 lines. n = 5 mice per genotype from three independent experiments. Error bars represent the mean ± SEM. Means with an asterisk (*) are significantly different from the corresponding control group (p < 0.05) according to F-protected post hoc analysis.
(D) Results of ISHH labeling of IL-1R1 of WT DG sections. The black arrow points to dendritic fields. The yellow arrow points to granule-cell-layer neurons. The arrowhead points to mossy fibers. The dashed square marks the area shown at higher magnification on the bottom right. Scale bar, 50 μm.
(E) A representative image of RFP labeling of Vglut2-Cre-Il1r1r/r DG sections. The white arrow points to dendritic fields. The yellow arrow points to granule-cell-layer neurons. The arrowhead points to hilar mossy fibers. Scale bar, 50 μm.
(F) A representative Tile Scan image of HA labeling of Il1r1GR/GR hippocampal sections. The dashed square marks the area shown at higher magnification on the bottom right. Scale bar, 200 μm.
(G) A representative Tile Scan image of RFP labeling of Camk2a-Cre-Il1r1r/r hippocampal sections. The dashed square marks the area shown at higher magnification on the bottom right. Scale bar, 100 μm.
(H) A representative Tile Scan image of RFP labeling of Tie2-Cre-Il1r1r/r hippocampal sections. The dashed lines indicate the outline of the hippocampus proper and choroid plexus. The dashed square marks the area shown at higher magnification on the bottom right. Abbreviation are as follows: CA, Cornu Ammonis; GCL, granule cell layer; ML, molecular layer; H, hilus; and CP, choroid plexus. Scale bar, 200 μm.
(I) Representative images of RFP and CD31 labeling in Tie2-Cre-Il1r1r/r brain sections. The dashed square marks the area shown at higher magnification on the bottom right. Scale bar, 100 μm.
(J) A representative Tile Scan image of RFP labeling of Gfap-Cre-Il1r1r/r hippocampal sections 24 h after an ICV IL-1β injection. The dotted lines indicate the outline of the hippocampus proper. The dashed square marks the area shown at higher magnification on the bottom right. Scale bar, 200 μm.
(K) Representative images of RFP and GFAP labeling of Gfap-Cre-Il1r1r/r brain sections. The dashed square marks the area shown at higher magnification on the bottom right. Scale bar, 50 μm. See also Figure S1.