Fig. 2.
A, Comparison of the numbers of primary spheres generated from different neurogenic regions in the adult mouse brain. The data are expressed as the mean (+SEM) number of spheres generated per 10,000 cells plated (i.e., per well; density = 20 cells per microliter). Tissue from each region (aLV, anterior lateral ventricle; pLV, posterior lateral ventricle; 3rd V, third ventricle;HV, hippocampal arch; DG, dentate gyrus) was dissociated into single cells and plated in serum-free media containing EGF + FGF2 and B27 supplement; spheres were counted at 7 DIV. Note that the aLV cells generate neurospheres at a rate ∼120-fold higher than the DG cells (0.54 ± 0.1), on the basis of data from n > 120 animals and 10 separate experiments. B, Comparison of the number of spheres generated from adult dentate gyrus at different rostrocaudal levels. Rostral, middle, and caudal sections through the DG yield a similar number of sphere colonies. Tissue from each individual 500 μm section (n = 85 sections from >20 animals) through the DG was separately dissociated into single cells and cultured at 20 cells per microliter; spheres were counted at 7 DIV.