Fig. 2.
HIF-1α immunostaining in the cerebral cortex at 1 d of recovery from cardiac arrest. A, HIF-1α was not detected in sham-operated rat brain. B, Positive HIF-1α immunostaining (green) in the brain cortex at 1 d of recovery in cells with rounded nuclei characteristic of neurons. C, D, Double staining for HIF-1α (green) and the neuronal-specific marker NeuN (red) showed partial colocalization. In addition, flat nuclei associated with small blood vessels stained for HIF-1α, probably corresponding to endothelial cells (C, arrow). Scale bar, 100 μm.