Fig. 2.
cle-1 and nid-1 gene structures and mutations. A, The cle-1gene structure is shown with the different domains indicated in the three isoforms as reported previously (GenBank AF164959) (Ackley et al., 2001). CLE-1A-specific exons are white, those in forms A and B are light gray, and those common to all three isoforms are dark gray, with the exceptions that exon 8 is unique to CLE-1B and exon 14 is unique to CLE-1C. The ju34 deletion removes part of exon 17, which encodes the Gly-X-Y collagenous domain, and causes a premature stop codon. The cg120 deletion removes exons 18–20 and also causes a premature termination. B, Thenid-1 gene structure is shown with the globular and rod domains indicated. The structure of nid-1 and thecg119 and cg118 deletions have been reported previously (Kang and Kramer, 2000). The cg119deletion removes exons 1–7 of the nid-1 coding region plus 948 bp upstream of the ATG and is a molecular null for NID-1. Thecg118 deletion is in frame and removes exons 2–8, resulting in an NID-1 protein missing some of the G1 and all of the G2 domains, leaving the rod and G3 domain intact.