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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Nat Protoc. 2019 Mar 4;14(4):1027–1053. doi: 10.1038/s41596-019-0126-x

Figure 5.

Figure 5.

Comparison of the AF4 performance for separating EVs using different membranes: regenerated cellulose (RC, red) versus poly(ether)sulfone (PES, blue). B16-F10 sEVs were analyzed using the following AF4 parameters: channel flow rate, 1.0 mL/min; a linear gradient of cross-flow decreasing from 0.5 mL/min to 0 mL/min over 45 min; channel height, 490 μm; sample focus time, 2 min; input amount, 40 μg. Top, QELS at 100°; bottom, UV absorbance at 280nm.