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. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Nat Protoc. 2019 Mar 4;14(4):1027–1053. doi: 10.1038/s41596-019-0126-x

Figure 4.

Figure 4.

Effect of the focus time upon AF4 fractionation. Shown are AF4 fractionation profiles of B16-F10 sEVs collected using a sample focus time of 2 min (red), 5 min (blue) or 10 min (black). Top, QELS at 100°; bottom, UV absorbance at 280nm. The other AF4 parameters are: channel flow rate, 1.0 mL/min; a linear gradient of cross-flow decreasing from 0.5 mL/min to 0 mL/min over 45 min; channel height, 490 μm; membrane, regenerated cellulose (RC), input amount, 40 μg.