Figure 3.
Emergence of CTB-labeled sensory axons beyond the rostral MSC graft-host interface. A, CTB-labeled sensory axons regenerate beyond the graft-host interface and enter rostral host white matter after combined administration of intraganglionic cAMP and axonal application of NT-3. The dashed line indicates the graft-host interface. g, Graft; h, host. B, Sensory axons extend for short distances beyond the rostral graft-host interface in subjects that receive axonal application of NT-3 within and beyond the lesion site, without cAMP injection into the DRG. However, the extent of growth in this group is modest. C, D, Sensory axons fail to regenerate in other groups, including recipients of cAMP injections alone into the DRG (C), recipients of NT-3 alone into the lesion site (D), or recipients that receive neither cAMP nor NT-3 (data not shown). Scale bar: (D) A-D, 35 μm.