Skip to main content
. Author manuscript; available in PMC: 2020 May 2.
Published in final edited form as: Mol Cell. 2019 Mar 19;74(3):508–520.e4. doi: 10.1016/j.molcel.2019.02.015

Figure 6.

Figure 6.

LNP-circRNA characterization. A) Cryo-TEM image of LNP-circRNA. B) hEpo expression 24 hours after transfection of 293 cells with equimolar quantities of LNP-5moU-mRNA or unmodified LNP-circRNA (left) and hEpo expression stability over 3 days (right; data presented as mean+SD, n=3). C) RIG-I and IFN-β1 transcript induction 24 hours after transfection of A549 cells with LNP-5moU-mRNA or unmodified LNP-circRNA. TR: transfection reagent plus 200ng RNA (MessengerMax); LNP(1x): 200ng LNP-RNA; LNP(2x): 400ng LNP-RNA; L: 5moU-mRNA; C: circRNA (data presented as mean+SD, n=3, ns=not significant, p<0.05). D) SEAP expression 48 hours after transfection of TLR reporter cells with the RNAs indicated in c), relative to null controls (data presented as mean+SD, n=3). E) Serum hEpo expression 6 hours after injection of 1.5 picomoles of LNP-5moU-mRNA or unmodified LNP-circRNA into visceral adipose (data presented as mean+SD, n=5 Linear 5moU, Circular; n=3 Mock). F) Relative hEpo expression in serum over 42 hours after injection with LNP-RNAs (data presented as mean+SD, n=5 Linear 5moU, Circular; n=3 Mock; see also Figure S6).