Figure 2.
ApoE4 induces translocation of HDAC4 and HDAC6 to the nucleus. SH-SY5Y cells were treated with cholesterol (Chol; 100 μm), ApoE3 (20 nm), ApoE4 (20 nm), ApoE3 (20 nm) + Chol (100 μm), and ApoE4 (20 nm) + Chol (100 μm) for 24 h. Cytosol and nuclear fractions were prepared for immunoblot. A, Immunoblot showing expression levels of HDAC4 and HDAC6 in cytosol and nuclear fractions of SH-SY5Y cells. M, Molecular weight marker. B, C, HDAC nuclear translocation reported as the percentage of total protein in the nucleus [nucleus/(cytosol + nucleus)]. ApoE4+Chol treatment caused a significant increase in nuclear translocation of HDAC4 and HDAC6 compared with ApoE3+Chol treatment. Data are reported as mean ± SEM of 3 independent experiments (Student's t test, *p < 0.05 and**p < 0.005).