Figure 3.
HFS activates synthesis of reporter protein. A1, B1, Fluorescence images of pyramidal cells transfected with reporter construct. Left baseline fluorescence, middle, and right color-coded changes from baseline fluorescence before (middle) and 60 min after (right) HFS (A1) or with continued baseline recordings (B1). Colors indicate the following: decrease, blue; no change, green; increase, red. Scale from −50 to +50%. A2, B2, Time course of fluorescence levels in ROIs from a cell subjected to HFS (A2) and a control cell without HFS (B2). ROIs are indicated in A1 and B1. A3, B3, Ensemble average of reporter protein levels from ROIs as defined in Figure 2B. Signals from secondary apical dendrites (distant from the stimulation electrode) that exhibited decreasing fluorescence levels after HFS were pooled together and represented by gray symbols in A3. Scale bars, 70 μm.