Figure 7.
Embryonic and early postnatal expression of nrp-1 and sema3A genes in the cortex. A, Mouse P0 (a), P10 (b), P15 (c), and 4-week-old (d) sagittal sections processed for in situ hybridization with probe for Sema3A and NRP-1. Cortical layers are shown on the left. Scale bar, 100 μm. MZ, Marginal zone; CP, cortical plate; tr, nerve fiber tract; VZ, ventricular zone. B, Immunoblot analysis of P0, P10, P15, and 4-week-old brain lysates with anti-Sema3A antibody and anti-NRP-1 antibodies. In general, Sema3A and NRP-1 were detectable in NP-40-soluble lysates of P0 to 4-week-old brains. To detect Sema3A in the brain at the developmental stage of P0, the pellets obtained were dissolved in 2× the sample buffer and further sonicated (Insoluble).