Figure 6.
GSK3β activity and APP metabolism in hAPP/DN–GSK3β double tg mice. A, Levels of GSK3β activity in immunoprecipitated samples from the frontal cortex of nontg, hAPP tg, DN–GSK3β tg, and hAPP/DN–GSK3β double tg mice. B, Levels of GSK3β activity in PP1γ-treated immunoprecipitated samples from the frontal cortex of nontg and DN–GSK3β tg mice. C, Semiquantitative immunoblot analysis of levels of GSK3β-p in immunoprecipitated samples from the frontal cortex of nontg and DN–GSK3β tg mice. D, Aβ1–42 levels in homogenates from the frontal cortex of nontg, hAPP tg, DN–GSK3β tg, and hAPP/DN–GSK3β tg mice. E, Immunoreactivity levels of the Aβ-cleaving enzyme neprilysin in homogenates from the frontal cortex of nontg, hAPP tg, DN–GSK3β tg, and hAPP/DN–GSK3β tg mice. F, Levels of BACE1 immunoreactivity in homogenates from the frontal cortex of nontg, hAPP tg, DN–GSK3β tg, and hAPP/DN–GSK3β tg mice. *p < 0.05 compared with hAPP tg mice by unpaired two-tailed Student's t test; n = 6 mice per group. **p < 0.05 compared with samples that were not treated with PP1γ by unpaired two-tailed Student's t test; n = 6 mice per group.