Figure 2.
Levels of Pt-(GG) DNA adducts in different cells types of the peripheral and CNS of mice after a single dose of cisplatin (2 or 10 mg/kg, i.p.). Cryosections from DRGs or spinal cord tissues were immunostained as in Figure 1 and integrated fluorescence signals from individual nuclei (blue, DNA; green, Pt-[GG]) (see Fig. 1) were measured by fluorescence microscopy-based quantitative image analysis. Adduct-related signals were normalized for the actual DNA content of each cell and expressed as AFUs. Columns represent mean values (±SEM) from each 200 cells. A, Comparison of peak adduct levels 24 h after drug injection. B, Repair kinetics in DRG cells after a dose of 10 mg/kg.