Figure 2.
Expression of parkin R275W mutant in flies promotes dopaminergic neuronal degeneration in select clusters. A, Representative confocal microscopy images showing TH-positive (red) dopaminergic neurons in the PPL1 cluster of 20-d-old control (CTRL) and parkin null flies (PK−/−) as well as in transgenic flies expressing wild-type (WT) or mutant parkin species, as indicated. B, Bar graph showing the number of dopaminergic (DA) neurons in different clusters of the various fly species, as depicted by different shades shown in the figure (*p < 0.05, **p < 0.01, Student's t test; n = 10). C, Representative confocal microscopy images showing TH-positive (red) dopaminergic neurons in the PAM cluster of 20-d-old control and parkin null flies (PK−/−) as well as in transgenic flies expressing normal or mutant parkin species, as indicated. PAM cluster in top row (boxed) are shown at higher magnification in corresponding bottom row (genotype: Ddc-Gal4/+; UAS-hParkin/+, control Ddc-Gal4/+)