Figure 7.
Axonal degeneration after OGD is not mediated by ionotropic glutamate receptors at P3 and P7. Slices from P3 and P7 mice were perfused with aCSF containing NBQX (30 μm) and/or MK-801 (10 μm) under normoxic conditions, or 1 h before the onset of 1 h OGD, and continuing through the reperfusion period. YFP+ axon morphology in P3 WM after normoxic perfusion (A), drug-free OGD (B), OGD plus NBQX (C), and OGD plus MK-801 (D). NBQX and MK-801 did not prevent axon beading after OGD. Scale bar, 25 μm. E, F, Quantitative analysis demonstrating no decrease in axon damage after OGD reperfusion in the presence of NBQX, MK-801, or both. Error bars represent SEM.