Figure 2.
Quantification by ELISA/luminometry of the expression levels of HA-tagged CaV3.2 channel CAE SNPs. A, B, The histograms represent relative light unit (RLU) normalized toward the nonpermeabilized WT signal (100% in A). The various CAE SNPs are presented as by Vitko et al. (2005). One construct harboring two SNPs, G773D and R788C (Chen et al., 2003b; Vitko et al., 2005), is labeled Double. A, Surface expression measured in nonpermeabilized condition. B, Total expression measured after Triton X-100 permeabilization. C, Ratio of surface/total expression identifies an increase in membrane expression for all but G499S SNP. D, Same results as in C except presented as fold increase over the control (WT). Statistically significant differences are indicated (*p < 0.05; **p < 0.01; ***p < 0.001).