Figure 8.
Effects of aging on WT and PK-KO glial cultures. Glial cultures of WT and PK-KO mice were maintained in DMEM plus 15% FCS for different periods of time. A, Immunodetection and densitometric analysis of astroglial (GFAP) protein by Western blot of young (1–3 months) and aged (6–9 months) WT and PK-KO glial cultures. Percentage of microglial cells (B) (Cd11b+) and proliferating (BrdU+) (C) cells present at 1–3 and 6–9 months of culture. D, GSH levels in young and aged WT and PK-KO glial cultures. Protein expression (E) and densitometric analysis (F) of HSP-70 protein in young and aged WT and PK-KO glial cultures. Percentage of microglial cells and GSH levels are expressed as the mean ± SEM from six replicates of four and two independent cultures, respectively. Values for Western blotting experiment are the mean ± SEM from four replicates of four independent cultures. Statistical analysis was performed by two-way ANOVA (the interaction between genotype and treatment and genotype and aging were p < 0.05), followed by Bonferroni's post hoc test. +p < 0.05; ++p < 0.01; +++p < 0.001, PK-KO versus WT cultures. ***p < 0.001 old versus young cultures.