Figure 2. Identification of hGFAP-GFP classification and astrocyte-enriched transcription factors revealed by scRNA-seq.
(A) Immunolabeling for GFAP and GS in the cortex of P5 hGFAP-GFP mice.
(B) The percentage of indicated cells among hGFAP-GFP+ cells in P5 mouse cortices (n=4 for GFAP; n=3 for GS and PDGFRα).
(C) Immunolabeling for GFAP, Olig2, and Slc1a3 from P5 hGFAP-GFP mice.
(D) Zoom on boxed area in panel C.
(E) The percentage of Olig2+ and Olig2– cells among hGFAP-GFP+GFAP+ (left) or hGFAP-GFP+Slc1a3+ (right) cells in P5 mouse cortices (n=3).
(F) Immunolabeling of Blbp in the cortices from hGFAP-GFP mice at P3.
(G) Expression of PDGFRα in the cortices of P5 hGFAP-GFP mice.
(H) Immunolabeling for Ppp1r14b and Olig2 in the cortices of hGFAP-GFP mice at P3.
(I) Immunolabeling for Olig2 and Ki67 from P5 hGFAP-GFP mice.
(J) Left, enlarged images of panel I show cells co-labeled with Ki67 (arrows) and cells without Ki67 (arrowheads). Right, percentage of Olig2+ and Olig2– cells among Ki67+ hGFAP-GFP+ double positive cells (>300 cell counts from 3 cortices).
(K) Immunolabeling for Olig2 and GFAP or Slc1a3 in adult cortices at P60.
(L) The percentage of GFAP+ or Slc1a3+ among Olig2+ cells at P5 and P60 (>400 cell counts from 3 cortices at each stage).
(M) Immunolabeling for BHLHE40 in P5 hGFAP-GFP cortices.
(N-P) Immunolabeling for N) PRDM16 and GS in P14 mouse spinal cord and O) BHLHE40 and GS in human cortices. DAPI, blue. Arrows; co-labeled cells. P) Percentage of BHLHE40+ or PRDM16+ in GS+ cells (>300 cell counts from 3 samples).
Data are presented as means ± SEM. Scale bars, 50 μm in A, C, G, I, K, M; 20 μm in F, N, O; 10 μm in D, H, J and G (inset).