Figure 5.
Increased neuronal excitability in APdE9 mice. A, Resting membrane potential (VR) of L2/3 pyramidal cells in APdE9 and wild-type mice at the age of 6 months. B, C, Incrementing current injection in the soma of L2/3 pyramids leads to premature action potential (AP) firing in APdE9 mice at subthreshold current steps compared with neurons from wild-type mice as early as 3.5 months of age. D, Representative recording traces. E, Schematic illustration of the L1 afferent stimulation paradigm. The inset shows a representative experimental setting. The bar graph indicates the relative distance of stimulating pipettes along the apical tuft of pyramidal cells from their somata. F, Representative recordings with incrementing extracellular stimulus strengths at 3.5 months of age. G, Cumulative analysis of neuronal excitability in response to afferent stimulation in APdE9 (n = 8) and wild-type (n = 6) slices. *p < 0.05, **p < 0.01 versus wild type.