Figure 6.
Parkin accelerates the assembly of 26S proteasome. A, Purified 26S proteasome was incubated with GST, GST-fused wild-type parkin, GST-fused parkin77-465, or GST-fused parkin1-170 for 1 h at 37°C. The samples were resolved on a native PAGE gel and subjected to immunoblotting with anti-20S core and anti-Rpt2 antibodies. B, The graph represents the band intensity of 26S proteasome measured by the ImageJ program (National Institutes of Health). Data represent the mean ± SEM of four independent experiments. ***p < 0.001 versus control sample incubated with GST.