Figure 3.
Age-dependent decrease in synaptophysin in APPE693Δ-Tg mice. A–L, Brain sections of 4 (A, E, I)-, 8 (B, F, J)-, 12 (C, G, K)-, and 24 (D, H, L)-month-old Tg mice were stained with antibody to the presynaptic marker synaptophysin. All images were taken from the hippocampal CA3 region. Unlike non-Tg littermates (A–D) and APPWT-Tg mice (E–H), the APPE693Δ-Tg mice (I–L) exhibited age-dependent decrease in synaptophysin in the hippocampus from 8 months. Scale bars, 30 μm. M, Synaptophysin fluorescence intensity in 30 μm × 60 μm area of the hippocampal CA3 region was quantified using the NIH ImageJ software and shown in arbitrary units (AU). Each bar represents the mean ± SEM (n = 3). *p = 0.0258 versus NonTg; p = 0.0244 versus APPWT-Tg, **p = 0.0052 versus NonTg; p = 0.0092 versus APPWT-Tg, ***p = 0.0140 versus NonTg; p = 0.0387 versus APPWT-Tg.