Figure 8.
GSLs impair starvation-induced degradation of APP-CTFs. H4 cells were incubated in EBSS for the indicated time periods, and the different proteins [APP-CTFs; full-length APP (APP-fl)] in cell lysates were detected by Western immunoblotting. EBSS (con), Incubation in EBSS in the absence of GSLs. EBSS+GSLs (+p), Incubation in EBSS in the presence of GSLs after pretreatment of cells with GSLs (50 ng/μl) for 48 h. EBSS + GSLs (−p), Incubation in EBSS in the presence of GSLs without pretreatment. Quantification was done by ECL imaging, and trend lines were plotted to understand the time-dependent metabolism of individual protein. Note that the initial amount of each protein was wet to 100%.