Table 5.
Watermaze: probe trial long-term memory (LTM) quantification
| Less-excit (α6Cacna1a) | WT, n = 20 | KO, n = 10 | MW/t test |
| Resting (time, %) | 6.2 ± 1.7 | 8.2 ± 3.0 | 0.72 |
| Speed (cm/s) | 12.4 ± 0.4 | 11.9 ± 0.6 | 0.53 |
| Pearson χ2 | |||
| Target quadrant (time, %) | 47.6 ± 3.2 | 43.9 ± 5.8 | 0.47 |
| Right quadrant (time, %) | 14.8 ± 1.8 | 17.7 ± 3.2 | 0.48 |
| Opposite quadrant (time, %) | 18.6 ± 1.8 | 18.8 ± 3.6 | 0.67 |
| Left quadrant (time, %) | 19.1 ± 2.4 | 19.7 ± 3.1 | 0.31 |
| ANOVA time in different quadrants | 0.00 | 0.00 | |
| No-inhib (L7-γ2) | WT, n = 10 | KO, n = 11 | MW/t test |
| Resting (time, %) | 10.3 ± 3.9 | 30.8 ± 7.9 | 0.07 |
| Speed (cm/s) | 11.4 ± 0.6 | 8.7 ± 1.0 | 0.03 |
| Pearson χ2 | |||
| Target quadrant (time, %) | 29.7 ± 4.9 | 28.5 ± 5.4 | 0.40 |
| Right quadrant (time, %) | 26.8 ± 3.7 | 27.8 ± 5.3 | 0.52 |
| Opposite quadrant (time, %) | 27.6 ± 4.5 | 24.1 ± 4.0 | 0.33 |
| Left quadrant (time, %) | 15.9 ± 3.3 | 19.7 ± 3.3 | 0.40 |
| ANOVA time in different quadrants | 0.10 | 0.52 | |
| No-LTP (L7-PP2B) | WT, n = 10 | KO, n = 10 | MW/t test |
| Resting (time, %) | 3.2 ± 0.7 | 15.7 ± 4.6 | 0.01 |
| Speed (cm/s) | 13.9 ± 0.5 | 11.1 ± 0.6 | 0.001 |
| Pearson χ2 | |||
| Target quadrant (time, %) | 32.9 ± 3.7 | 32.5 ± 3.3 | 0.40 |
| Right quadrant (time, %) | 28.2 ± 3.2 | 23.7 ± 3.4 | 0.38 |
| Opposite quadrant (time, %) | 21.6 ± 3.3 | 22.7 ± 3.4 | 0.45 |
| Left quadrant (time, %) | 17.4 ± 3.1 | 21.2 ± 3.5 | 0.40 |
| ANOVA time in different quadrants | 0.01 | 0.10 | |
| No-LTD (L7-PKCi) | WT, n = 22 | TG, n = 22 | MW/t test |
| Resting (time, %) | 23.8 ± 3.9 | 19.1 ± 3.6 | 0.29 |
| Speed (cm/s) | 9.9 ± 0.6 | 10.0 ± 0.6 | 0.91 |
| Pearson χ2 | |||
| Target quadrant (time, %) | 47.6 ± 4.4 | 46.9 ± 4.5 | 0.43 |
| Right quadrant (time, %) | 17.7 ± 2.5 | 19.8 ± 3.0 | 0.37 |
| Opposite quadrant (time, %) | 18.8 ± 3.1 | 18.9 ± 3.8 | 0.33 |
| Left quadrant (time, %) | 16.6 ± 2.6 | 14.5 ± 2.7 | 0.55 |
| ANOVA time in different quadrants | 0.00 | 0.00 | 0.00 |
Mean values ± SEM of the percentage of time spent resting, the average swimming speed and the percentage of time spent in the four different quadrants (target, right, left, and opposite) during the 60 s probe trial given 2 weeks after the previous probe trial for the four mouse lines. Statistical differences between genotypes (KO/TG vs WT) were calculated with a Student's t test for normally-distributed data (regular font) or with a Mann–Whitney test for non-normally distributed data (MW; italic font), and with a Pearson χ2 for the time spent in different zones. Successfulness of the test (i.e., most of time spent in quadrant target) was calculated per genotype with an ANOVA test.