Figure 3.
Localization of MCT4 in the PNS. A, Cultured rat SCs were lipofected with scrambled or MCT4 siRNA. MCT4 localized to the perinuclear cytoplasm (arrowheads), and the signal was abrogated when MCT4 expression was knocked down, demonstrating both the presence of the transporter and the specificity of the antibody. B, Immunostaining on cross section from adult mouse sciatic nerve revealed the presence of MCT4 in the endoneurium (e). C, D, MCT4 is expressed in myelinated teased fibers in a thin line and a mesh-like shape (arrowheads), suggesting localization in the outer cytoplasmic mesaxonal line and Cajal bands. MCT4 expression is also visible in perinuclear cytoplasm (arrow). E, Cross section of sciatic nerve reveals the presence of MCT4 in the perinuclear cytoplasm (arrows) and in the most abaxonal part (arrowheads) of myelinated SCs. F, MCT4 appears as a discontinuous patchy pattern at the most abaxonal part of the fiber (arrowheads), above the myelin sheath stained by MBP. Scale bars: A, C, D, 20 μm; B, 50 μm; E, F, 10 μm. P, perineurium.