Figure 2.
Localization of MCT1 in the PNS. A, The MCT1 blocking peptide abrogates the MCT1 staining on teased fibers. Both images are shown with the same intensity level. B, Immunostaining on cross section from adult mouse sciatic nerve shows the presence of MCT1 in both perineurium (p) and endoneurium (e). C, Immunostaining on cross section from adult mouse DRG revealed that MCT1 is particularly enriched in the “capsule” (arrowheads) and at the surface of soma of a subset of sensory neurons. D, MCT1 is strongly present at the SLI, colocalizing with phalloidin (arrowheads). E, MCT1 colocalizes with Caspr indicating its presence in paranodal regions (arrowheads). Inserts show a magnification of the nodal and paranodal region. Scale bars: A, C–E, 20 μm; B, 50 μm.