Table 1.
Antibodies used, their source, and working concentrations
| Antigen | Antibody | Source | Concentration |
|---|---|---|---|
| Alix | 3A9 | Cell Signaling Technology | 1 μg/ml |
| Flotillin-1 | 18/Flotillin-1 | BD Biosciences | 1 μg/ml |
| HIV glycoprotein 120 | 46-4 | ATCC | 1 μg/ml |
| PrP | ICSM35 | D-Gen | 0.5 μg/ml |
| β3-tubulin | ab68193 | Abcam | 1:250 dilution |
| β3-tubulin | TUJ1 | Covance | 5 μg/ml |
| Tau (aa 6-18) | Tau12 (human) | EMD Millipore | 0.1 μg/mla |
| Tau (aa 7-13) | HJ9.4 (rodent) | Gift from David Holtzman | 1 μg/mla |
| Tau (aa 194-198) | BT2 (human + rodent) | Thermo Scientific | 1 μg/mla,b |
| Tau ( aa 210-241) | Tau5 (human + rodent) | Cell Signaling Technology | 1 μg/mlc |
| Tau (aa 243-441) | K9JA (human + rodent) | DAKO | 1 μg/mla,b,c |
| Tau (aa 404-441) | Tau46 (human + rodent) | Cell Signaling Technology | 1 μg/mla |
| Misfolded tau (residues 7-9 + 313-322) | MC1 | Gift from Peter Davies | 5 μg/mla |
Concentrations used for Western blotting:
a 5 μg/ml for IP,
b 2.5 μg/ml for ELISA,
c 5 μg/ml for ELISA.