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. Author manuscript; available in PMC: 2019 Jun 28.
Published in final edited form as: Nature. 2018 May 2;557(7704):247–251. doi: 10.1038/s41586-018-0075-5

Figure 2: Hepatocyte-derived peripheral cholangiocytes are equivalent to normal mature peripheral cholangiocytes.

Figure 2:

a-c, IF of hepatocyte-fate-traced P120 Alb-Cre+/−;Rbpjf/f;Hnf6f/f;R26ZG+/+ mouse liver (n=3 each). Scale bars, 100 μm (c), 20 μm (a, b). d-g, RNA-seq analysis of normal peripheral cholangiocytes (pC, n=3 mice), hepatocyte-derived peripheral cholangiocytes (HpC, n=4 mice) and RBPJ- and HNF6-deficient hepatocytes (H, n=3 mice). Principal-component analysis (d). Venn diagram showing number of genes significantly differentially up- and down-regulated in pC or HpC vs. H (e). Heatmaps of genes reflecting cholangiocyte differentiation, including genes lacking in DDC diet-induced hepatocyte-derived metaplastic biliary cells (top) and other marker genes (bottom) (f). Heatmaps of genes reflecting hepatocyte differentiation, including all differentially expressed CYP genes enriched in adult mouse liver (top) and other marker genes (bottom) (g). 1-way ANOVA, FDR-corrected P<0.05; fold change > 3 (e-g). 2-sided Student’s t-test; bold genes P<0.05 for HpC vs. pC (f, g).