Figure 2.
Dysfunctional insulin signaling is induced by the treatment of neurons with iron. Changes of in tyrosine phosphorylation, and total IRβ, IRS-1, and PI3K p85α protein, were evaluated using Western blot (A–C). Antibodies recognizing IRβ (G) or IRS-1 (J) were utilized for co-immunoprecipitation with total proteins, and then the predicated proteins were determined using Western blot. (D–F) Show the quantitation of the results presented in (A–C), respectively. (H,I) Show the quantitation of the results presented in (G). (K,L) Show the quantitation of the results presented in (J). Results are shown as Mean ± SD. Student's t-test was employed to determine the significance. NS, no significant difference.