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N-glycosylation of neurexophilin. Neurexophilin complexed to neurexin Iα was purified by affinity chromatography on immobilized α-latrotoxin and incubated with or without PNGase F. Samples (0.5 μg protein) were analyzed by immunoblotting with antibodies to neurexophilin. Numbers on the right indicate positions of molecular weight markers.
