Fig. 5.
Double-staining of neurons for tubulin (A, C, E, G) and tau (B, D, F, H) in cells processed according to staining condition 2. A–D show low- and high-magnification views of cells processed using the combined fixation and extraction procedure of Lee and Rook (1992) (procedure 3).E–H show low- and high-magnification views of cells fixed without extraction according to procedure 4. Both procedures reveal that tau and tubulin staining are more intense in the distal part of the axon compared to its more proximal regions (see text for additional details), and that tau colocalizes with MTs in the growth cone (seeC, D, G, and H and Fig. 8). Scale bar inA = 56 μm and indicates scale in all low-magnification views (A, B, E, F). Scale bar in C = 13 μm and indicates scale in all high-magnification views (C, D, G, H).