Fig. 6.
Isolation, cloning, and characterization of rapsyn-like cDNAs derived from ciliary ganglia. A, Ganglionic cDNAs were reamplified using the nested primer pair (S2/A2), uncovering three products at 1350, 1250, and 1000 bp (lane 2) on ethidium-stained agarose gels. As in Figure 4, no products were detected when RT was omitted from the initial reverse transcription reaction (lane 3). Three of the cDNAs were subcloned (CG7, CG11, and CG4) and displayed insert sizes appropriate to the corresponding PCR products (lanes 4-6, respectively). B, Sequencing cDNA inserts from each of the ganglionic clones. Only nucleotides within and surrounding exon 2 (boxed region bordered by inverted Ts) are depicted, with dashes indicating nucleotides missing from CG11 or CG4 numbering above CG7 indicating the corresponding position in Ch43K.1 (see Fig.2B). Amino acid residues encoded by CG7 and missing from CG11 or CG4 are shown in one letter code below CG4, with leucine, isoleucine, and methionine (leucine alternatives) (see Landschulz et al., 1988; Froehner, 1991) residues inboxes.