Fig. 1.
Identification of GABAA receptor subunits in affinity-purified recombinant α1β3γ2 receptors. HEK 293 cells were transfected with α1β3γ2 subunits. Receptors were extracted, purified by affinity chromatography, and subjected to SDS-PAGE and Western blot analysis by using the digoxigenated subunit-specific antibodies and chemiluminescence detection, as indicated. Chemiluminescence exposure time was varied to result in comparable signal intensities for the individual antibodies. Antibody mix (Ab-Mix): a mixture of anti-peptideα1(1–9), anti-peptide β3(1–13), and anti-peptide γ2(319–366) antibodies was used for the detection of GABAA receptor subunits. The experiment was performed three times with similar results.