Figure 5. Renin lineage and aquaporin 2 expressing cells in non-diabetic and diabetic Ren1d-Cre;mT/mG mouse kidneys (A) and colocalization analysis (B, C).
A: Kidney sections from Ren1d-Cre;mT/mG mice show the presence of GFP+ collecting duct epithelial cells. Immunofluorescence for AQP2 show colocalization with GFP+ collecting duct cells in both diabetic and non-diabetic Ren1d-Cre;mT/mG mice. B: Colocalization analysis of GFP+ cells and immunofluorescence for AQP2 cells using Pearson’s correlation coefficient shows no difference between kidneys of control and diabetic Ren1d-Cre;mT/mG mice. C: A large image analysis of the GFP and AQP2 colocalized area per total area shows no significant difference between kidneys of control and diabetic Ren1d-Cre;mT/mG mice. AQP2: aquaporin 2; STZ: streptozotocin-treated mice; n=5 in each group;