Figure 4.
Caveolin‐1 silencing reduced oxLDL‐induced cell death. Caveolin‐1 silencing shifted oxLDL‐induced cell apoptosis. SMC/cav1 (A) or human primary VSMC (passage 5) (B) were either transfected with 100 nM caveolin‐1 siRNA (siRNA cav1) or with 100 nM scrambled siRNA (siRNA sc) then serum starved, and incubated with oxLDL (100 μg ApoB/ml) for 18 hrs. Expression of caveolin‐1 protein was analysed by immunoblotting using an anti‐caveolin‐1 antibody as described under ‘Materials and methods’ and cell viability was evaluated by the MTT assay. Results are expressed as percentage of untreated control and represent the mean ± S.E.M. of three independent experiments (*P < 0.05).