Figure 2. NLRP12 suppresses RIG-I-mediated IFN-β, ISRE and NF-κB reporter activities.
HEK293 cells were transfected with 100 ng of (A) IFN-β, (B) ISRE or (C) NF-κB luciferase reporter with the internal control Renilla luciferase reporter pLR-TK plasmid and indicated plasmids (RIG-I, TRIM25, MAVS, STING or TRAF3) in the presence of empty vector (EV, pCDNA3) or NLRP12-encoding plasmid (pCDNA3/HA-NLRP12, 30, 100, 300 ng/sample). Luciferase assays were performed 24 hr post-transfection. (D) HEK293 cells were transfected with IFN-β luciferase reporter (30 ng), pLR-TK (3ng), and pFlag-CMV2-RIG-I plasmid (300 ng) or pFlag-CMV2 (Vector) with EV or NLRP12 expression plasmid (300 ng). 5’ppp-dsRNA and LMW poly(I:C) were then transfected into these cells at 24 hr, and luciferase assays were performed 12 hr later. Data were collected and expressed as mean ± s.e.m. from at least three independent experiments. Student’s t test was performed, *P<0.05; **P<0.01; ***P<0.001 for EV versus NLRP12 transfectants.