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. Author manuscript; available in PMC: 2020 Feb 21.
Published in final edited form as: Cell Chem Biol. 2018 Dec 27;26(2):300–306.e9. doi: 10.1016/j.chembiol.2018.11.006

Figure 3. BSJ-03–123 exploits homolog-selective dependencies.

Figure 3.

(A) Bottom: Waterfall plot of 391 cell lines ranked by CDK6 dependency as determined in genome-wide CRISPR/Cas9 screens. CERES essentiality score is normalized for copy number variation and scaled by setting the median of pan-essential genes to −1. Top: mRNA levels of CDK4, CDK6 and D-type cyclins. (B) Colony formation assays (12 days, refreshing the treatment every 2 d). (C) Growth curves of AML cell lines treated with 200 nM BSJ, BSJ-bump or DMSO. Cells were counted and treatment refreshed every 2 days. (D) Cell cycle after treatment with 200 nM BSJ or BSJ-bump for 24 h. (E) Percentage of apoptotic cells after 24 h treatment with 200 nM palbociclib, BSJ, BSJ-bump or DMSO (Caspase-Glo® 3/7 assay). BET protein degrader dBET6 served as positive control. (F) Growth curves of CDK4-deficient MV4–11 treated with 200 nM BSJ, palbociclib or DMSO. See also Figure S2.