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. Author manuscript; available in PMC: 2020 Jan 24.
Published in final edited form as: Cell. 2019 Jan 17;176(3):581–596.e18. doi: 10.1016/j.cell.2018.12.012

Figure 5. XBP1 in astrocytes controls monocytes and microglia during EAE.

Figure 5.

A) Model of XBP1+ astrocyte pro-inflammatory signaling in the CNS. B) Flow cytometry plots (left) and quantification (right) of pro-inflammatory monocytes in CNS during EAE. n=10 per group. Unpaired two-tailed t-test. C) Pathway analysis of monocyte RNA-seq data. Columns are n=2 biological replicates. D) Heatmap of monocyte RNA-seq data (p<0.05). E) Gene set enrichment analysis of monocyte pathways. F) Heatmap of microglia RNA-seq data (p<0.05). n=4 shScrmbl, n=3 shXbp1. (G and H) Gene set enrichment analysis and pathway analysis of microglia RNA-seq. Ct=Gfap::shScrmbl, KD=Gfap::shXbp1. I) Nanostring analysis of EAE microglia from 4I. RNA pooled from n=5 mice, n=7 for sgXbp1. See also Tables S4–S5.